The action of hyaluronan in functional properties, morphology and expression of matrix effectors in mammary cancer cells depends on its molecular size

Breast cancer constitutes a heterogeneous disease. The expression profiles of estrogen receptors (ERs), as well the patterns extracellular matrix (ECM) macromolecules, determine its development and progression. Hyaluronan (HA) is an ECM molecule that regulates breast cells' properties in molecular size-dependent way. Previous studies have shown 200-kDa HA fragments modulate functional properties, morphology, several mediators highly metastatic ERα−/ERβ+ MDA-MB-231 cells. In order to evaluate effects (< 10, 30 200-kDa) ERβ-suppressed cells, shERβ cells were used. These are less aggressive when compared with To this end, molecules associated potential studied. Notably, both cell proliferation invasion significantly reduced after treatment HA. Moreover, assessed by scanning electron microscopy, affected cellular qPCR, upregulated epithelial marker Ε-cadherin. mediators, such HAS2, CD44, MMP7, also altered. On other hand, migration levels syndecan-4 (SDC-4) not contrast our observations regarding novel data demonstrate size determines on exhibits antiproliferative these A deeper understanding mechanism may contribute therapeutic strategies against cancer.